Pasteurella is a genus from the family of Pasteurella. It is a gram negative, coccobacillus,facultatively anaerobic bacteria that exhibit a distinct characteristics of bipolar staining when gram stained which is far different from all other bacteria on earth. It is non-motile and pleomorphic and often catalase and oxidase positive. It constitutes part of the normal Flora of the nose and mouth in dogs, cats and other animals.
Pasteurella multocida is highly sensitive to enrofloxacin, oxytetracycline, chloramphenicol. It grows at 37 degrees on blood agar but not on macConkey.
How to culture Pasteurella
1) Prepare a blood agar ( this getting a nutrient agar add mixing it with blood). Note that some antibiotics like chloramphenicol should be used to depress growth if non-existant fastidious organisms because Pasteurella is a fastidious organisms and then to be overpowered by organisms like Staphylococcus aureus.
2) Do a sterility check for the agar to ensure that it’s not contaminated by putting the solidified agar in a petri-dish and incubating for 24 hours at 37 degrees in an incubator.
3) After the 24 hours source a sample from the nasal area of a dog or cat using a swab stick.
4) Using the spread style of culturing spread over the blood agar using the swab stick containing your samples
5) Incubate for 24 hours under 37 degrees.
6) The next day view your cultures
7) To confirm gram stained and view on the slide if you see coccobacillus exhibiting bipolar staining the it’s Pasteurella.
Pastuerella is a pathogenic organism and one of the causes of pneumonia in cats and dogs.